A selective, accurate, precise and reproducible high performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of glycopyrrolate in tablet dosage form (glycopyrrolate tablet USP). The separation was performed on pre-coated silica gel 60 F254 (Merck) plate using ethyl acetate: water: glacial acetic acid (7:2.0:1.0 v/v/v) as mobile phase. Densitometric analysis was performed in reflectance-absorbance mode at 222 nm. Rf value of the glycopyrrolate was found to be 0.34. The linear regression analysis data for the calibration plot showed good linear relationship with correlation coefficient 0.99 (1-6 µg/band). The limit of detection and quantitation were found to be 0.220 µg/ml and 0.667 µg/ml respectively. The percentage recovery of glycopyrrolate was found to be 99.87%. The statistical parameters and recovery study were carried out and reported.
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